Researchers blend folk treatment, high tech for promising anti-cancer compound

Ancient wisdom, technological savvy -


Researchers at the University of Washington have blended the past with the present in the fight against cancer, synthesizing
a promising new compound from an ancient Chinese remedy that uses cancer cells' rapacious appetite for iron to make them a
target.


The substance, artemisinin, is derived from the wormwood plant and has been used in China since ancient times to treat
malaria. Earlier work by Henry Lai and Narendra Singh, both UW bioengineers, indicated that artemisinin alone could
selectively kill cancer cells while leaving normal cells unharmed.


The new compound appears to vastly improve that deadly selectivity, according to a new study that appeared in a recent issue
of the journal Life Sciences. In addition to Lai and Singh, co-authors include Tomikazu Sasaki and Archna Messay, both UW
chemists.


"By itself, artemisinin is about 100 times more selective in killing cancer cells as opposed to normal cells," Lai said. "In
this study, the new artemisinin compound was 34,000 times more potent in killing the cancer cells as opposed to their normal
cousins. So the tagging process appears to have greatly increased the potency of artemisinin's cancer-killing properties."



The compound has been licensed to Chongqing Holley Holdings and Holley Pharmaceuticals, its U.S. subsidiary, to be developed
for possible use in humans. Although the compound is promising, officials say, potential use for people is still years away.



In the study, researchers exposed human leukemia cells and white blood cells to the compound. While the leukemia cells
quickly died, the white blood cells remained essentially unharmed.


The trick to the compound's effectiveness, according to Lai, appears to be in taking advantage of how cancer cells function.



Because they multiply so rapidly, most cancer cells need more iron than normal cells to replicate DNA. To facilitate that,
cancer cells have inlets on their surface, known as transferrin receptors, in greater numbers than other cells. Those
receptors allow quick transport into the cell of transferrin, an iron-carrying protein found in blood.


In creating the compound, researchers bound artemisinin to transferrin at the molecular level. The combination of the two
ingredients appears to fool the cancer cell.


"We call it a Trojan horse because the cancer cell recognizes transferrin as a natural, harmless protein," Lai said. "So the
cell picks up the compound without knowing that a bomb - artemisinin - is hidden inside."


Once inside the cell, the artemisinin reacts with the iron, spawning highly reactive chemicals called "free radicals." The
free radicals attack other molecules and the cell membrane, breaking it apart and killing the cell.


According to Lai, that process is what initially piqued his interest in artemisinin about 10 years ago. The wormwood extract
was used centuries ago in China, but the treatment became lost over time. In the 1970s, it was rediscovered as part of an
ancient manuscript containing medical remedies, including a recipe that used a wormwood extract. The medical community soon
discovered that the extract, artemisinin, worked well against malaria, and it is currently used for that purpose throughout
Asia and Africa.


Artemisinin combats malaria because the malaria parasite collects high iron concentrations as it metabolizes hemoglobin in
the blood. As science began to understand how artemisinin functioned, Lai said, he began to wonder if the process had
implications for cancer treatment.


"I started thinking that maybe we could use this knowledge to selectively target cancer cells," he said. "So far, the outlook
appears good."


The next step in development under the Holley licensing agreement will likely be testing in animals and, if that pans out,
human trials to gauge the compound's effectiveness. The current study was funded by the Artemisinin Research Foundation and
Chongqing Holley Holdings.


For more information, contact Lai at (206) 543-1071 or hlaiu.washington. The Holley contact is Michael Liu at (714)
606-8415 or michaelholleypharma.


The article is available on-line at sciencedirect/science. Click the "journals" button and look under Life Sciences, Volume
76, Issue 11. The article is No. 9 on the Web page (page 1267-1279).


Rob Harrill - rharrillu.washington

University of Washington